FESTKÖRPER NMR DISSERTATION
Refubium – Structure determination of immobilized proteins by solid-state NMR spectroscopy
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Create lists, bibliographies and reviews: The study of bacterial membrane proteins within structural genomics projects is an obvious next application of the novel technique described herein, together with the study of receptor-bound agonists and antagonists. Find a copy online Links to this item Volltext Volltext Volltext kostenfrei. In Chapter 7, the structural information contained in the chemical-shift was exploited by analysing the differences in solution and solid-state NMR chemical-shifts of the SH3 domain.
You already recently feztkörper this item. Write a review Rate this item: Add a review and share your thoughts with other readers. The main focus of this thesis is the development of a strategy for the collection of a large number of distance restraints for structure determination. The objectives of this research are to establish the molecular origin of the catalytic activity of this supported Re complex including the structures of adsorption and reaction sites and their relationship to its activity for olefin metathesis.
In particular, shift differences provided insight into contacts between SH3 molecules in the micro-crystalline preparation used. Please enter your name. The unique virtue of solid- state NMR lies in its ability to study biomolecules without size restrictions and without the need of large well-ordered crystals.
The first instance addresses the question of the activation of a grafted organometallic rhenium complex by virtue of its interaction with a particular support, namely amorphous silica-alumina. Your rating has been recorded.
View all subjects More like this Similar Items. Remember me on this computer. In the second part of this work the effect of rather different interfacial interactions are examined, namely those of structural changes following fstkörper treatments on the optical properties of nanoscale Group III-V semiconductors and their ternary analogs.
Please verify that you are not a robot. Please choose whether or not you want other users to be able to see on your profile that this library is a favorite of yours. This leads to the 71Ga species being located in chemically and electronically more ordered molecular environments near the nme of the nanoparticle, and hence improved photoluminescence.
The spectroscopic technique we chose for the collection of structural-defining 13CC distances is the proton-driven spin diffusion PDSD recoupling method. The resonances of non-exchangeable protons were assigned by 3D 1HCC correlation spectroscopy.
Structure determination of immobilized proteins by solid-state NMR spectroscopy. Would you also like to submit a review for this item?
Journal for Facility Management. Please re-enter recipient e-mail address es. The general outline of the method is presented in Chapter 3. Synthesis of this catalytic system is accomplished by chemical vapor deposition onto the support. These issues are no longer insurmountable hurdles that once deemed solid-state NMR a rather exotic method for structure determination. Please enter the message. The biosynthetically site-directed 13C-enriched sissertation needed for the measurement of long-range constraints were obtained by growing bacteria on [C] glycerol and [1,C] glycerol.
Structure determination of immobilized proteins by solid-state NMR spectroscopy
This thesis demonstrates the forthcoming role of solid-state MAS NMR as a mainstream technique for structural investigation of proteins, as a method complementary to solution NMR and single-crystal diffraction. The E-mail message field is required. A simple method was derived to extract distance information from build-up curves of cross-peak intensities in 15NN PDSD spectra. An almost complete 13C and 15N resonance assignment of a uniformly 13C- and 15N-labelled SH3 sample was achieved previously Pauli et al.